This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034028) and human (NP_009005) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity:Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Coronin-1A (Coronin-like protein A) (Clipin-A) (Coronin-like protein p57) (Tryptophan aspartate-containing coat protein) (TACO)

Target Description: Human Coronin 1a is a 51,026 dalton protein (461 amino acids) selectively expressed in cells involved in immune surveillance throughout the body, including activated microglial cells of the CNS. It is a member of the WD40 (tryptophan-aspartate) gene family, which includes the beta subunit of the trimeric G proteins as the prototype. Its function is presumably in membrane structural reorganization accompanying antigen-presentation.

Gene ID: CORO1A CORO1

Antibody Registry ID (RRID): AB_2313539

Physical State: Liquid

Production Notes: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a sequence shared between the human (NM_007074) and mouse (NM_009898) gene products. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Validation and Application Notes

Molecular Weight: 51 kDa

Western Blot Dilution Range: 1:5000-1:10000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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