Details

ChickenswereimmunizedwithActinpurifiedfromrabbitmuscle.Afterrepeatedinjections,immuneeggswerecollected,andtheIgYfractionswerepurifiedfromtheyolks.TheseIgYfractionswerethenaffinity-purifiedusingapeptidecolumn.Thefinalproductcontains50%glycerol(v/v),purifiedanti-ActinIgYfraction(10mg/ml)spikedwith25µg/mlofaffinitypurifiedanti-ActinIgY,andthenfilter-sterilized.

Volume:100µL(ACT-1010)or400µL(ACT-1040)

Concentration:10mg/mL

Clonality:Polyclonal

HostSpecies:Chicken

SpeciesReactivity:Human,Mouse,Rat

Applications:WB

AntibodyRegistryID(RRID):AB_2313504

PhysicalState:Liquid

Buffer:Sodiumphosphate(10mM,pH7.2)bufferedisotonicsaline(0.9%,w/v),glycerol(50%,v/v),withsodiumazide(0.02%,w/v)asananti-microbialagent.

ValidationandApplicationNotes

MolecularWeight:42kDa

QualityControl

Thisanti-ActinIgYpreparationwasanalyzedbyWesternBlotanalysis(atadilutionof1:2000)usingHRP-labeledgoatanti-chickenIgY(1:5000dilution,AvesLabsCat.#H-1004)asthesecondaryreagent.

Storage

Storeat-20˚Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Donotfreezeantibodiesat-80˚C,unlessyouwanttostorethemforlongerperiodsoftime.Notethateachtimeanantibodypreparationisfrozenabouthalfitsbindingactivityislost.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

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