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主营:抗体、抗鸡、免疫沉淀、神经细胞标记、二抗、阻断试剂
℡ 4000-520-616
℡ 4000-520-616
Aves Labs/Anti-Green Fluorescent Protein Antibody (GFP)/100 µL (GFP-1010)
产品编号:100µL(GFP-1010)
市  场 价:¥4500.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗猪>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$225.00
品      牌: Aves Labs
公      司:Aves Labs, Inc.
公司分类:
Aves Labs/Anti-Green Fluorescent Protein Antibody (GFP)/100 µL (GFP-1010)
商品介绍

Volume: 100 µL (GFP-1010) or 400 µL (GFP-1020)

Concentration: 10 mg/mL

Clonality: Polyclonal

Form: IgY Fraction

Host Species: Chicken

Applications: ELISA, ICC, IHC, WB

Target Description: Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Antibody Registry ID (RRID): AB_2307313

Physical State: Liquid

Buffer: Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Production Notes: Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).

Validation and Application Notes

Western Blot Dilution Range: 1:5000-1:10000

IHC Dilution Range: 1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product.Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Since 50% glycerol is present in the vial, this antibody preparation should remain a liquid at -20°C. For longer storage periods, store at -80°C, but be aware that freezing this preparation may reduce its activity.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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品牌介绍
Aves Labs是一家成立于上世纪九十年代,专注于高亲和性鸡源抗体的研发和准备,生物医药和生命科学行业全球*的鸡多克隆抗体(IgY)及定制抗体/多肽/免疫试剂的专业公司。Aves Labs在鸡源神经系统标志蛋白/抗体研究领域拥有独特优势,不仅品种多样,质量优良,而且适用于人/大小鼠等多个物种的神经系统研究,包括神经元/胶质细胞/雪旺细胞的各亚细胞结构免疫双标/免疫共沉淀研究。公司独有的技术及优质抗体产品有助于全球科研人员更为便捷的解决学术研究及应用研发课题。除了丰富的产品线外,Aves Labs还为客户提供优质的抗体定制服务,您只需要提供需要研究的重组蛋白或合成多肽,我们即可为您生产优质的鸡源多克隆抗体。
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