Volume: 1 mL

Concentration: 1 mg/mL

Clonality: Polyclonal

Form: Affinity Purified

Host Species: Chicken

Species Reactivity: Goat

Immunogen: IgG

Physical State: Liquid

Conjugate: HRP

Buffer: HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), thimerosal (0.01%, w/v) (as an anti-microbial agent).

Specificity: There is no detectable cross-reactivity with rabbit, human or mouse IgG

Production Notes: Chickens were immunized with purified, non-denatured goat IgG (containing both heavy and light chains). Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG’s were affinity-purified over a column conjugated with purified goat IgG (containing both heavy and light chains) and then conjugated with horseradish peroxidase.

Quality Control

Antibodies were tested for specificity using double-immunodiffusion in agarose gels and ELISA analysis. In the latter, a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using tetramethylbenzidine as substrate.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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