Details

GoatswereimmunizedwithpurifiedchickenIgY(wholeprotein)emulsifiedinFreund’sadjuvant.Aftermultipleinjections,goatswerebledandserumcollected.Antibodieswereisolatedusingaffinitychromatographyandconjugatedtoalkalinephosphatase.

Volume:1mL

Concentration:0.1mg/mL

Clonality:Polyclonal

Form:AffinityPurified

HostSpecies:Goat

SpeciesReactivity:Chicken

Immunogen:IgY

PhysicalState:Liquid

Conjugate:AP

Buffer:HEPES(50mM,pH7.0),NaCl(0.1M),bovineserumalbumin(0.2%,w/v),MgCl2(1.0mM),ZnCl2(0.1mM),sodiumazide(0.02%,w/v)(asanantimicrobialagent).

Specificity:Doesnotcross-reactwithmammalianIgGs

QualityControl

Antibodieswereanalyzedusingimmunoelectrophoresis(seebelow)andasandwichELISA.Inthislattertest,a1:800dilutionofGGHL-30PH-2wasfoundtogenerateanO.Dof1.0ina20minutereactionwithp-nitrophenylphosphateasthesubstrate.

Storage

Storeat4°Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Forlongerstorageperiods,dilutetheantibodieswithsterileglycerolandstoreat-20°C.Donotfreezetheseantibodiesunlessyouwanttostorethemforlongerperiodsoftime.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

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